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KMID : 0370219870310020064
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Yakhak Hoeji
1987 Volume.31 No. 2 p.64 ~ p.69
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Enzyme Immunoassay for Human Chorionic Gonadotropin Using Monoclonal Antibodies
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차상훈/Cha SH
김학주/김원배/양중익/Kim HJ/Kim WB/Yang JI
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Abstract
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Monoclonal antibodies against human chorionic gonadotropin (hCG) were prepared and characterized by examining isotype, epitope binding, cross reactivity and affinity constants. And a sandwich type enzyme immunoassay for native hCG was developed with solid phase monoclonal antibody against the conformational determinant expressed only on native hCG and horseradish peroxidase conjugated monoclonal antibody against the beta-subunit of hCG. The assay was sensitive to 1mIU hCG/ml and shown a linear response up to 200mIU hCG/ml. The cross reactivity for luteinizing hormone and beta-subunit of hCG were 1% and 0.18%, respectively.
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KEYWORD
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